Generator

Part:BBa_K902048:Design

Designed by: Emily Hicks, Stephanie Willatt   Group: iGEM12_Calgary   (2012-10-02)

Constitutive XylE generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 399
    Illegal NgoMIV site found at 571
    Illegal AgeI site found at 922
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Both the tetR promoter and the XylE gene were from the 2012 kit plates.

References

1Shu L, Chiou Y, Orville AM, Miller MA, Lipscomb JD, Que L. X-ray absorption spectroscopic studies of the Fe(II) active site of catechol 2,3-dioxygenase. Implications for the extradiol cleavage mechanism. Biochem 1995; 34:6649-6659.